https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 Simultaneous adsorption and biodegradation (SAB) of diesel oil using immobilized Acinetobacter venetianus on porous material https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:24636 80%). Acinetobacter venetianus immobilized on MBC demonstrated superior efficiency in degrading diesel oil (94%) compared to planktonic cells culture (82%) over a 3-day period. Moreover, the simultaneous adsorption and biodegradation of diesel oil using these immobilized cells fitted well to the pseudo second order (R² > 0.99). Scanning electron microscopy (SEM) revealed that through absorption, cells attached well to the cavum of MBC stalk cells. Fourier transform infrared analysis (FTIR) revealed that a large number of bands at 1300-1500 cm-1 existed, demonstrating that the diesel oil was degraded and new bands were formed. Gas Chromatography Mass Spectrum (GC-MS) analysis indicated the immobilized cells could degrade diesel oil into esters and aldehydes. Results justified the applicability of MBC as the carrier matrix for immobilizing microorganisms in removing diesel oil compounds from industrial wastewater.]]> Tue 14 Nov 2023 13:37:32 AEDT ]]> Soil bacterial strains with heavy metal resistance and high potential in degrading diesel oil and n-alkanes https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26714 Pseudomonas sp., Pseudomonas putida TPHK-1 and Pseudomonas aeruginosa TPHK-4, were more efficient in degrading high concentrations of the hydrocarbons than the other two strains, Stenotrophomonas maltophilia TPHK-2 and Acenitobacter sp. TPHK-3. P. putida TPHK-1 exhibited tolerance to very high concentrations of heavy metals such as cadmium, lead, zinc and copper. The innate ability of P. putida TPHK-1, as evidenced by the amplified genes alkB1 and alkB2 that encode alkane hydroxylases, and cat12o and cat23o coding for catechol dioxygenase, in degrading diesel oil in the presence of heavy metals is far greater than that of the strains reported in the literature. Heavy metal tolerance coupled with rapid degradation of hydrocarbons, even at high concentrations, suggests that P. putida TPHK-1 has a great potential in remediating soils contaminated with mixtures of hydrocarbons and heavy metals.]]> Sat 24 Mar 2018 07:26:23 AEDT ]]> Remediation of water contaminated with diesel oil using a coupled process: biological degradation followed by heterogeneous Fenton-like oxidation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32675 Acinetobacter venetianus in 96 h, while the removal efficiency of chemical oxygen demand (COD) in the aqueous phase was only 56.8%, indicating that degraded metabolites existed in solution. To solve this problem, a Fenton-like system consisting of nanoscale zero-valent iron (nZVI) and hydrogen peroxide was used for further oxidation of the metabolites after biodegradation. Results showed that the total COD removal increased from 56.8% to 89% under the optimal condition. In addition, effects of initial pH (2.0–9.0), ZVI dosage (0–2.0 g L-1), hydrogen peroxide (H₂O₂) dosage concentration (0–15 mmol L-1) and temperature (298–308 K) on the treatment efficiency of the combined process were studied. Scanning electron microscopy (SEM) demonstrated that changes to the surface of nZVI occurred. GC-MS revealed that the degraded metabolites were mineralized practically by nZVI/H₂O₂ system. The results points towards the potential of Fenton-like oxidation as a short post-treatment after a biological process for the treatment of organic pollutants in wastewater.]]> Fri 17 Nov 2023 11:42:05 AEDT ]]>